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Title: Detection of mixed Leishmania infections in dogs from an endemic area in southeastern Brazil
Title of periodic: Acta Tropica
Authors: Souza, Natália Alves
Leite, Rodrigo Souza
Silva, Soraia de Oliveira
Penna, Michele Groenner
Vilela, Liza Figueiredo Felicori
Melo, Maria Norma
Andrade, Antero Silva Ribeiro de Antero Silva
Affiliation: Centro de Desenvolvimento da Tecnologia Nuclear, CDTN, Belo Horizonte, MG, Brasil
Fundacao Ezequiel Dias FUNED, Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais, UFMG, Belo Horizonte MG, Brasil
Universidade Federal de Minas Gerais, UFMG, Belo Horizonte MG, Brasil
Issue Date: 2019
Keywords: Conjuctival swab;Diagnosis;Canine Visceral leishmaniasis
Abstract: In Brazil, the visceral leishmaniasis (VL) is caused by Leishmania infantum, while the tegumentary leishmaniasis (TL) etiological agents are mainly Leishmania braziliensis and Leishmania amazonensis. The canine visceral leishmaniasis (CVL) diagnosis is an important step of the VL control program in Brazil, which involves the elimination of infected dogs, the main urban VL reservoirs. The current serology-based diagnostic tests have shown cross-reactivity between these three species, whereas molecular diagnosis allows high sensitivity and specie identification. In the present study, 349 dogs of the metropolitan region of Belo Horizonte (Minas Gerais state) were screened by conjunctival swab and the samples analyzed by ITS-1 nested PCR. Thirty dogs (8.5%) tested positive. The RFLP of amplicons using HaeIII demonstrated that 17/30 samples presented a banding pattern compatible with L. infantum, 4/30 matched with L. amazonenis, 1/30 with L braziliensis and 8/30 showed a mixed infection pattern. The samples that were distinct of L. infantum or presented a mixed pattern were submitted to RFPL with HaeIII and RsaI enzymes that confirmed the mixed pattern. Such patterns were also confirmed by Sanger Sequencing. The results pointed eight dogs with mixed infections and the establishment of TL, causing species in the Belo Horizonte dog population. These findings highlight the need for more comprehensive epidemiological studies, since the TL transmission profile might be changing. This study also shows the potential of the ITS1-nPCR associated with RFLP for the proper Leishmania diagnosis and typing in the dog population.
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Appears in Collections:Artigo de periódico

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