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Title: Modifying internal organization and surface morphology of siRNA lipoplexes by sodium alginate addition for efficient siRNA delivery
Title of periodic: Journal of Colloid and Interface Science
Authors: ARRUDA, Danielle Campio
GONZALEZ, Ismael Jose
FINET, Stephanie
TRICHET, Valerie
ANDRADE, Gracielle Ferreira
BIGEY, Pascal
Affiliation: Universidade Federal de Minas Gerais, UFMG, Belo Horizonte MG, Brasil
CNRS, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), UMR, Paris, France
INSERM, UTCBS U 1022, F-75006 Paris, France
Université Paris Descartes, Sorbonne-Paris-Cité University, UTCBS, F-75006 Paris, France
Chimie ParisTech, PSL Research University, UTCBS, F-75005 Paris, France
Centro de Desenvolvimento da Tecnologia Nuclear, CDTN, Belo Horizonte, MG, Brasil
CNRS, Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), UMR 7590, F-75005 Paris, France
Sorbonne Université, IMPMC, F-75005 Paris, France
IRD, IMPMC, F-75005 Paris, France
MNHN, IMPMC, F-75005 Paris, France
University of Chile, Santiago, Chile
Issue Date: 2019
Keywords: Nanoparticle;Polymers
Abstract: Vectorized small interfering RNAs (siRNAs) are widely used to induce specific mRNA degradation in the intracellular compartment of eukaryotic cells. Recently, we developed efficient cationic lipid-based siRNA vectors (siRNA lipoplexes or siLex) containing sodium alginate (Nalg-siLex) with superior efficiency and stability properties than siLex. In this study, we assessed the physicochemical and some biological properties of Nalg-siLex compared to siLex. While no significant differences in size, ζ potential and siRNA compaction were detected, the addition of sodium alginate modified the particle morphology, producing smoother and heterogeneous particles characterized by transmission electron microscopy. We also noted that Nalg-siLex have surface differences observed by X-ray photoelectron spectroscopy. These differences could arise from an internal reorganization of components induced by the addition of sodium alginate, that is indicated by Small-Angle X-ray Scattering results. Moreover, Nalg-siLex did not trigger significant hepatotoxicity nor inflammatory cytokine secretion compared to siLex. Taken together these results suggest that sodium alginate played a key role by structuring and reinforcing siRNA lipoplexes, leading to more stable and efficient delivery vector.
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Appears in Collections:Artigo de periódico

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