Leishmania (Viannia) braziliensis is the prevalente species infecting patients with tegumentary leismaniasis from Mato Grosso state, Brazil

Abstract

The frequency of Leishmania (Viannia) braziliensis infection among patients of Mato Grosso, Brazil, was estimated by polymerase chain reaction-PCR, DNA hybridization and by isoenzyme electrophoresis. Analysis of DNA polymorphism was carried out using random amplified polymorphic DNA-PCR (RAPDPCR) with five different primers. The patients were attended from May 1997 to February 1998 at the Reference Ambulatory for American Tegumentary Leishmaniasis at Júlio Mueller Hospital of the Federal University of Mato Grosso, Brazil.