Please use this identifier to cite or link to this item: http://www.repositorio.cdtn.br:8080/jspui/handle/123456789/437
Title of periodic: Mycoses Berlin
Authors: Demicheli, Marina Cortez
Reis, B.S.
Goes, A.M.
Andrade, Antero Silva Ribeiro de
Affiliation: Centro de Desenvolvimento da Tecnologia Nuclear/CDTN, Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais/UFMG, Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais/UFMG, Belo Horizonte, MG, Brasil
Centro de Desenvolvimento da Tecnologia Nuclear/CDTN, Belo Horizonte, MG, Brasil
Issue Date: 2006
Keywords: Fungi;gamma radiation;vaccines
Abstract: Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent mycosis in Latin America, and currently there is no effective vaccine. The aim of this study was to attenuate the yeast form of P. brasiliensis by gamma irradiation for further studies on vaccine research. Paracoccidioides brasiliensis (strain Pb 18) cultures were irradiated at doses between 0.5 and 8.0 kGy. After each dose the viability, reproductive ability and protein metabolism were evaluated. The comparison between the antigenic profile of irradiated and control yeast was made by Western blot and the virulence evaluated by the inoculation in C57Bl/J6 mice. At 6.5 kGy the yeast lost its reproductive capacity. The viability and the incorporation of [L-35S]-methionine were the same in control and up to 6.5 kGy irradiated cells, but 6.5 kGy-irradiated yeast secreted 40 percent less proteins. The Western blot profile was clearly similar in control and 6.5 kGy-irradiated yeast. No colony-forming unit (CFU) could be recovered from the tissues of the mice infected with the radioattenuated yeast. We concluded that for P. brasiliensis yeast it is possible to find a dose in which the pathogen loses its reproductive ability and virulence, while retaining its viability, metabolic activity and the antigenic profile.
Access: L
Appears in Collections:Artigo de periódico

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